SYNTHESIS OF NAMROI POMELO PEEL ESSENTIAL OIL INWATER NANOEMULSION FOR ANTIBACTERIAL APPLICATION

Abstract

Nanoemulsion based on Pomelo essential oil extracted from Pomelo peels was successfully combined with nanosilver by ultrasonic vibration method. The most important factors affecting the nanoemulsion particle size such as ratio of two types of emulsifiers including Tween 80 and Span 80, the volume of Pomelo essential oil and water volume were also investigated in detail. Particle size distribution of nanoemulsion was investigated by Dynamic Light Scattering (DLS) method. Nanoemulsion samples were also tested for the resistance to Escherichia coli, Bacillus subtilis, Staphylococcus aureus and Salmonella enterica by disc diffusion method. The study results showed that the nanoemulsions based on Pomelo essential oil combined with nanosilver were nanosized in the range from 10 to 115 nm and capable of resisting to mentinoned above four types of bacteria superior to nanoemulsion based only on pure Pomelo essential oil.