ESTIMATION OF EC50 VALUES IN ACUTE TOXICITY TEST THROUGH SEVERAL STATISTICAL SOFTWARES

Abstract

The chemical toxicity test on crustaceans is a commonly used model to estimate 50% effect concentrations (EC₅₀) to identify potential risks to aquatic systems. The objective of the study was the apply statistical softwares in estimating EC₅₀ values for a laboratory model toxicity results. In the present study, the EC₅₀ value of the pesticide Dichloro-Diphenyl-Trichloroethane (DDT) during acute exposure (24h) to Moina macrocopa (M. macrocopa) was estimated using a dose-response curve and four statistical softwares such as JMP Pro 16, Origin Pro 8.5.1, Sigmaplot 14.0, IBM SPSS 20. The estimated EC₅₀ value when applying the dose-response curve is 25.06 (µg.L^–1), for statistical softwares is 13.48, 22.41, 25.70 and 22.55 (µg.L^–1), respectively. The EC₅₀ value is essentially statistically different with the biggest difference being the JMP Pro 16 software (~10 µg.L^–1). The different setup equation and data distribution pattern of each software lead to a different EC₅₀ values (standard error (SE) = 2.19 µg.L^–1) for a toxicity data. All statistical software obtained EC₅₀ values, but JMP Pro 16 analysis showed the best performance due to the ability to extract indicators not established in the regression model’s equation.