EVALUATION OF PROBIOTIC CHARACTERISTICS OF GRAM POSITIVE BACTERIA ISOLATED FROM DIGESTIVE TRACT OF CHICKENS

Abstract

Probiotic bacteria are widely used in food, pharmaceutical products and considered as one of the main components of livestock industry. Microbial sources with probiotic properties are abundant in nature, especially in poultry gastrointestinal tract. The study was conducted to isolate bacterial strains with probiotic properties from digestive tract of chickens. Among 50 isolated strains, 5 bacterum strains with good probiotic characteristics were selected, including C19, C31, C38, C43 and C47. These strains were able to growth in low pH condition, withstand bile concentration over 1%, weak or moderate antagonism with the pathogenic bacteria, and showed spore forming capable under heat treatment. Using sequencing of 16S rRNA region, the selected strains were indicated belong to Bacillus genus. Taken together, the results of this study showed the applicability of the probiotic bacteria isolated from chicken gastrointestinal tract for the poultry industry.