INVESTIGATION OF FLAVONOID, PHENOLIC CONTENTS AND THE ABILITY TO KILL BREAST CANCER CELL 4T1 OF BETEL LEAF EXTRACTS

Abstract

Breast cancer is a leading cause of cancer death in women. Plant-based active ingredients that can inhibit the growth of cancer cells can be used to replace or support treatment, besides chemotherapy, radiation and surgery measures. Betel leaves are used in folk remedies in Vietnam with many therapeutic effects. In this study, the extracts of ethanol, hexane and ethyl acetate from betel leaves will be investigated for their flavonoid, phenolic components and their ability to induce cytotoxicity against breast cancer cell-line 4T1. The results showed that the flavonoid content was 49.71, 37.40, 21.34 mg QE/g and the phenolic content was 684.23, 368.34, 318.82 GAE/g in ethanol, hexane, and ethyl acetate, respectively. At a concentration of 25 μg/ml, both ethanol and ethyl acetate extracts were highly toxic, killing most cancer cells, while having no effect on the growth ability of normal fibroblast cells.