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Nanoemulsion based on orange essential oil extracted from King orange peels was successfully synthesized by ultrasonic vibration combined with mechanical stirring. The chemical composition of the orange essential oil sample was determined by Gas Chromatography - Mass Spectometry (GC-MS) method. Particle size distribution of nanoemulsion was investigated by Dynamic Light Scattering (DLS) method. The most important technical factors affecting the size of nanoemulsion such as ratio of two types of emulsifiers including Tween 80 and Span 80, the volume of orange essential oil and the effect of water volume were also investigated in detail. Nanoemulsion samples were also tested for the resistance to E. coli by disc diffusion method. The study results showed that the obtained emulsion particles were nanosized in the range from 60 to 120 nm and after combining with nanosilver, the resulted nanoemulsion was capable of resisting E. coli superior to nanoemulsion based on pure orange essential oils.