ANTIBACTERIAL ACTIVITY OF SILVER NANOPARTICLES PRODUCED BY POLYOL METHOD AGAINST E.COLI, S.AUREUS AND P.AERUGINOSA

Abstract

Antibacterial activity of silver nanoparticles produced by polyol using EG as a reductant and PVP as a protectant against E.coli, S.aureus, and P.aeruginosa was studied. Nanoparticles were less than 20nm in size and stable after a period of 28 days. The sterilization efficiency was >99% with E.coli after 1 minute exposure at 25ppm of silver nanoparticle concentration; meanwhile it were >60% and >40% with P.aerugonisa and S.aureus after 5 minutes exposure at the concentration of 100ppm. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of silver nano particles on E. coli, S. aureus and P.aeruginosa were 3,125ppm, 12,5ppm, 6,25ppm and 6,25ppm, 25ppm, 12,5ppm, respectively. Silver nanoparticles that is produced using non-toxic reductant and having efficient antibacterial activity could be a source for biological medical application.