ISOLATION AND IDENTIFICATION OF RUMEN CELLULOLYTIC BACTERIA FOR AGRICULTURAL BYPRODUCT BIO-DEGRADATION

Abstract

Cow rumen microbes play an important role in cellulose digestion in animal nourishment. To select potential microorganisms for cellulose bio-degradation in agricultural byproducts, thirty-seven bacteria strains and four mold strains which were isolated from the cow rumen were identified and described charateristics and physiology of colony and cells. Among them, eighty bacteria strains and three mold strains showed cellulose-degrading activities on carbomethyl cellulose (CMC) medium. Five bacteria strains and two mold strains with highest of cellulose-degrading activities, due to hydrolyzed CMC halos diameters/colony diameter >2.5 for bacteria and >1.5 for molds, were selected for identify by 16S rRNA or ITS, respectively, gene sequencing. The results indicated that K9, Db1, T8, và Db7, N1 and N2 strains were Bacillus flexus, Bacillus megaterium, Bacillus subtilis, Acinetobacter calcoaceticus, Aspergillus versicolor và Aspergillus terreus, respectively, with similarity index at medium and high level.