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Microorganisms play an important role in the breakdown of organic compounds in nature and are the object of enzyme production in the world. In particular, amylase is one of the three largest used groups in the industrial enzymes, and it is always necessary to select high amylase-producing microorganisms that can work under extreme conditions. Notably, actinomycetes are the potential targets for industrial amylase production. In this study, 23 strains of actinomycetes were isolated from many different soil samples, of which RB-XK3 was selected and identified as Streptomyces canus. This strain showed high amylase biosynthesis in Gause II medium containing 1% starch substrate and 0.5% NH4NO3 with pH 8.0 at 37C for 96 hours. The RB-XK3 strain shows superior potential to produce amylase in an alkaline environment instead of the neutral one like other actinomycetes and thus can be applied in many different fields.