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Jasminum annamense subsp. annamense is a rare subspecies belonging Oleaceae family. Based on Molecular biology techniques, the ITS region of Jasminum annamense subsp. annamense was first successfully amplified and sequenced. The results also showed the differences in ITS sequence between J. annamense subsp. annamense and J. annamense subsp. glabrescens which are similar morphological characteristics. Furthermore, the present study found 7 compounds in ethanol extracts of leaf and stem of J. annamense subsp. annamense by using Liquid chromatography/mass spectrometry (LC/MS) analysis, including β-D-xylose, jasmolactone A, jasmolactone B, jasmolactone C, heptacosane, (Z)-8- dehydromelissic acid and 10 hydroxyloeuropein. Moreover, the results showed that ethanol extracts could inhibit the growth of 2 tested microogranisms, such as Bacillus cereus và Salmonella typhimurium.