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The integration of bacterial cellulose and other antibacterial material might produce a composite that can eliminate bacteria. Composite of bacterial cellulose and low molecular weight chitosan produced using ex situ method. Antibacterial activity of the composite was examined for Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Corynebacterium diphtheriae. Antibacterial property of chitosan was presented in the composite for all 4 bacterial strains. However, Gram negative bacteria were eliminatedmore efficiently than Gram positive bacteria in this study. While chitosan solution showed a weak bactericidal perfomance to S.aureus, it happened to be much stronger that the composite can kill this microorganism. Composite between chitosan and bacterial cellulose can be used to prevent and eliminate bacteria.