CHEMICAL COMPOSITION AND IN VITRO PROPAGATION OF NAM TINH PIERRE

Abstract

Nam tinh pierre (A. pierreanum Engl.) belongs to genus Arisaema, is a rare species. It has just only discovered in Ba Den mountain, Tay Ninh province. Many species in this genus were investigated about chemical composition and biological activities, with medicinal properties. Up to present, studies on Nam tinh pierre were only about classification. This current study aimed to implement chemical composistion analysis and in vitro propagation of the species.

Chemical composition in tuber of Nam tinh pierre was determined by alcohol extraction method, and then analyzed using liquid chromatography-mass spectrometry (LC-MS). The result of analysis showed 176 compounds and identified 11 compounds that have molecular weight similar to the compounds founded in genus Arisaema, including (2R*,3S*,5S*)-N,2-dimethyl-3-hydroxy-5(10-phenyldecyl)pyrrolidine; germacrene D; campesterol; emodin; coniferin; methylconiferin; 6-deacetylnimbin; 28-deoxonimbolide; aurantiamide acetate (N-benzoyl-1 -phenylalanyl-1 phenylalaninol acetate); luteolin; colchicine.

This is the first time in vitro propagation of Nam tinh pierre has been successful. Suitable time of sterilization of the fresh tuber using NaClO 5% was about 30 minutes. MS medium supplemented 2 mg/L 2,4-D was suitable for callus proliferation. MS medium supplemented with 1,5 mg/L BA was suitable for shoot formation from the callus. The ½ MS medium supplemented with 1,5 mg/L NAA and 0,2 mg/L BA was suitable for root formation form the in vitro shoot.