CULTURE CONDITIONS FOR CELLULASE PRODUCTION FROM Bacillus subtilis TH-VK22

Abstract

The majority of cellulase enzymes available today are derived from microorganisms. Given their capacity for fast growth and synthesizing of a wide variety of extracellular enzymes, bacteria in particular have emerged as a class of organisms with high potential for cellulase production and application. In this study, the Bacillus subtilis TH-VK22 strain was investigated for its capacity to produce cellulase in basal Bushnell Haas Medium medium (BHM) supplemented with different types of carbon and nitrogen sources as well initial pH, inoculation temperature and time. The results showed that growing of B. subtilis TH-VK22 in BHM medium supplemented with 11.0% maltodextrin, 2.0% peptone, and 0.5% rice straw, with an initial pH of 5.0, 48 hours of inoculation at 35°C gave rise to the highest cellulase production. In addition, B. subtilis TH-VK22 were shown to be able to produce high amount of cellulases in a wide range of initial pH from 5.0 to 9.0. This finding highlights B. subtilis TH- VK22 potential in a variety of sectors, including biofuel production, food processing, waste treatment and bioremediation.