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Composite of bacterial cellulose and silver nanoparticles was investigated for its antibacterial activities against 4 bacteria including E.coli, S.aureus, P.aeruginosa, and B.subtilis. The composite BC/AgNPs inhibited the growth of bacteria, exhibited through antibacterial zone of inhibition to all 4 species. Diameter of inhibition ring against Gram positive bacteria, including S.aureus and B.subtilis was larger than that of inhibition ring to against negative bacteria E.coli and P.aeruginosa (P<0.05). When bacterial liquid culture was directly contact with the composite, bactericidal performance up to 100% was detected after 15, 45, 60 minutes to E.coli, B.subtilis, and P.aeruginosa, respectively; meanwhile, S.aureus was compeletly removed after 120 minutes.