SYNTHESIS AND INVESTIGATION OF ANTIOXIDANT ACTIVITY OF TRYPTOPHILLIN L PEPTIDES

Abstract

Two Tryptophyllin L tetrapeptides including Phe-Pro-Trp-Leu(OH) and Phe-Pro-TrpLeu(NH2) were successfully synthesized using Fmoc solid-phase peptide synthesis technique. The structures of two peptides were confirmed by mass spectrometry (MS) and nuclear magnetic resonance (NMR) measurements. Purity of more than 95% for both peptides were revealed by Reverse Phase Liquid Chromatography (RP-HPLC). Screening for antioxidant activities of the two peptides by DPPH and FRAP assays showed a high antioxidant potency of Phe-Pro-Trp-Leu(NH2) which is in contrast with PhePro-Trp-Leu(OH) and required further investigation for its potential application as an antioxidant for domestic or medicinal purpose.