OPTIMIZING CONDITIONS TO YIELD TOTAL PHENOLIC CONTENTS USING ULTRASOUND-ASSISTED EXTRACTION FROM CASHEW TESTA (ANACARDIM OCCIDENTALE LINN.) TỐI ƯU HÓA CÁC ĐIỀU KIỆN CHIẾT XUẤT TỔNG HÀM LƯỢNG PHENOLIC TỪ VỎ LỤA HẠT ĐIỀU (ANACARDIUM OCCIDENTALE LINN.) CÓ SỰ HỖ TRỢ CỦA SIÊU ÂM

Abstract

The optimal experiments on cashew testa (Anacardim occidentale Linn.) were conducted with three factors of extraction (including liquid-solid ratio, extraction time, and extraction temperature), and the responses is total phenolic content (TPC). The result showed the highest of TPC at 375.73 mg of gallic acid equivalent/g dry weight (mg GAE/g DW) when the solvent is methanol, liquid-solid ratio at 42.6 (mL/g), extraction time at 97.8 (min), and extraction temperature at 48.5^oC. The quantitative analysis of five main components of cashew testa including Catechin, Epicatechin, Epigallocatechin gallate (EGCG), Epicatechin gallate in the seven locals in Vietnam was performed by HPLC. The results showed that Epicatechin (25.495-44.539 mg/g DW) was the highest content, following by Epicatechin gallate (7.492-13.501 mg/g DW), Catechin (2.625-4.279 mg/g DW), EGCG (1.609-2.168 mg/g DW), and caffein (1.335-1.168 mg/g DW).