ENHANCED PRODUCTION OF CELLULASE FROM Bacillus sp. D11

Abstract

Cellulase, an enzyme that hydrolyzes the glycosidic bonds in cellulose to form simple sugar molecules, is widely used in various industrial processes. The finding of new microbial strains producing extracellular cellulase as well as their cellulase biosynthesis conditions has been continuingly reported up to date. In this study, the influence of different factors such as substrate source, nitrogen source, initial pH, temperature, incubation time for cellulase biosynthesis of Bacillus sp. D11 has been identified. Bacillus sp. D11 showed strong cellulase biosynthesis with 450.49±12.03 UI/mL in the medium containing 2.0% rice straw (w/v), 1.0% earthworm powder (w/v), 1.0% NaCl (w/v), 0.5% yeast extract (w/v), initial pH 6.0 at 37℃, 150 rpm after 96 hours of incubation. By 16S-rRNA sequencing method, Bacillus sp. D11 was identified to be closely related to Bacillus amyloliquefaciens MT013383. Overall, the obtained results will contribute to the development of cellulase production processes and applications in different fields.