CLONING ERNS GENE OF CLASSICAL SWINE FEVER VIRUS – TOWARDS CREATION OF SUBUNIT VACCINE AGAINST CLASSICAL SWINE FEVER

Abstract

Classical swine fever virus (CSFV) is composed of 4 structural proteins, in which E^rns is an important virulence factor in the serological diagnosis and vaccine development. This study was conducted to generate E. coli lines carrying the full-length E^rns gene sequence and initially evaluated the feasibility of generating recombinant E^rns protein. Twenty-one E^rns gene sequences were collected, inserted into the plasmid, and transformed into E. coli competent cells. Plasmids from successfully inserted bacterial lines were extracted and sequenced to evaluate the effectiveness of the cloning method. Sequencing results show that the study has successfully created 21 lines of E. coli carrying the E^rns gene of field strains. These 21 sequences have very high similarity at nucleotides (88.99 - 98.38%) and amino acids (87.67 - 99.56%) levels to100 sequences having the highest similarity on NCBI