DNA BARCODE OF TWO CURCUMA SPECIES NEWLY DESCRIBED FROM VIETNAM

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VĂN HỒNG THIỆN
TRẦN ĐÌNH THẮNG
LÊ VĂN SƠN
NGUYỄN PHI NGÀ
LƯU HỒNG TRƯỜNG
TRẦN HỮU ĐĂNG
TRỊNH NGỌC NAM

Abstract

Curcuma xanthella and Curcuma cotuana were recently described as the new species for the flora of Vietnam. The present study successfully amplified and sequenced the ITS, trnL-F và matK regions of C. xanthella as well as provided for the first time, the sequences of ITS2, trnL-F and matK regions of C. cotuana. The results of this study helped to determine several ambiguous nucleotides on the GenBank ITS sequence of C. xanthella (Lý 348) specimen. In addition, based on these molecular markers, the present study also provided the differences in the ITS, trnL-F and matK sequences among these two species and the other Curcuma plants, C. singularis, C. flaviflora, C. vitelline and C. rhomba that were similar in morphological characteristics.

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Chemical, Bio, Food, Environmental Technology