ANALYSING ANTIOXIDANTS FROM LIMNOPHILA AROMATICA

Abstract

After drying, Limnophila aromatica was defatted with hexane and then extracted antioxidants
with methanol solvent. Time for fat removal and extraction were carried out at 1h, 2h, 3h. The crude extracts were calculated a yield and were determined total phenolic content (TPC) by Folin Ciocalteu method. The results showed that the time of fat removal only affects to yield and no effect to TPC. While, the
longer extraction time, the higher TPC. The M3-3, deffated and extracted for 3 hours, has highest yield
(8,51%) and highest TPC (44,25 mg GAE/g sample). Antioxidant activity of M3-3 was determined by
DPPH method. . These results show that Limnophila aromatica is as a
source of natural antioxidants.