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In the present work, three flavonoids and a stillbene were isolated from the methanol extract of the leaves of Andrographis paniculata B. with a combination of various chromatographic techniques, including silica gel, Sephadex LH-20 and RP-18. On the basis of spectroscopic data analysis, they were elucidated apigetrine (1); luteoloside (2); rutine (3); polydatine (4). Their structures were charaterized by 1D, 2D NMR and MS spectroscopies. The compounds are the first report on the chemical constituents of Vietnamese Andrographis paniculata B.