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Dendrobium anosmum Di Linh Taly originated from Di Linh District, Lam Dong Province is a precious orchid, of high economic value, and in great demand of collectors. Protocorm-like bodies (PLB) Dendrobium anosmum Di Linh Taly were investigated by in vitro culture under FL (flouresent lamp) and under light-emitting diodes with blue and red combination (LED BR 30:35). The in vitro regenerated plantlets were hardened under greenhouse environment for 1 month. Percentage of PLB producing shoots was 38 – 42% under LED 30B:35R, 3.8 – 4.2 times more than that under FL (10%). The number of roots per shoot under LED 30B:35R was 3.67 – 5.16, significantly more than that under FL (1.95 roots per shoot). Root length under LED 30B:35R was 3.42 – 4.14 mm while that under FL was 2.97 mm. The in vitro plantlets all were well acclimated under greenhouse environment. The LED 30B:35R could significantly promote growth and development of shoots and roots Dendrobium anosmum Di Linh Taly, increasing cost effectiveness in micropropagation.