ESTABLISH THE INITIAL PROCEDURE FOR DETECTING VIBRIO PARAHAEMOLYTICUS IN THE SHRIMP BY ONE STEP- PCR METHOD

Abstract

Early Mortality Syndrome (EMS), also known as Acute Hepatopancreas Necrosis Disease (AHPND) in shrimps, has been identified as being caused by a strain of V. parahaemolyticus. This disease has caused enormous damage to the shrimp farming industry among countries in the world in general and Vietnam in particular. One of the main reasons for uncontrollable widespread disease is the process of identifying pathogens is slow and inaccurate, leading to no promptly preventive measurement. In order to enhance the ability to briefly detect disease and improve the current disease status, in this study the PCR process using two specific 16S rRNA and ldh primers has been developed to swiftly diagnose V. parahaemolyticus pathogen in shrimps with a detection threshold of 3.5x103 CFU/ml