BIOLOGICAL ACTIVITY OF DOXORUBICIN IN COMBINATION WITH EXTRACTS OF FIVE ZINGIBERACEAE SPECIES

Abstract

Rhizomes of Zingiber officinale Roscoe (ZO), Zingiber zerumbet (L.) Roscoe ex Sm. (ZZ), Curcuma clovisii Škornič (CC), Curcuma zedoaria (Christm.) Roscoe, CZ) and fruit of Amomum villosum Lour (AV) were investigated for extraction by immersion with different organic solvents at room temperature and away from direct sunlight. The immersion was made with three different solvents, chloroform (TCM), ethyl acetate (AcOEt), and ethanol (EtOH), for periods of 1, 7, 7, 14, and 30 days. TCM and EtOH were significantly the best extracting solvents. Wild ginger rhizomes provided the highest yield of total extract (8.0%). The anticancer activity against HCT-116 cells and the cytotoxicity against the human skin fibroblasts HSF cells in vitro were studied following MTT-test. The result demonstrated that ginger extracts showed a good effect on the biological activity of doxorubicin. The chemical composition of obtained extracts were analyzed by using liquid chromatography/mass spectrometry (LC/MS), thin layer chromatography (TLC), column chromatography on silica gel.