ENHANCED PRODUCTION OF CELLULASE FROM Bacillus amyloliquefaciens D19

Abstract

Cellulase is an enzyme mainly produced by microorganisms, which hydrolyzes cellulose into monosaccharide and is widely used in environmental treatment and many different industrial sectors such as industrial paper, garment, food, ... In this study, we have found suitable culture conditions including substrate source, nitrogen source, carbon source, initial pH, and culture temperature for Bacillus amyloliquefaciens D19 cellulase biosynthesis cellulase biosynthesis. The results showed that after culturing this strain in medium supplemented with 0,5% straw, 1% soy flour (w/v), 1% NaCl (w/v), 0,5% yeast extract (w/v) in 48 hours at shaking conditions 150 rpm, 37°C, initial pH 5,0, the highest enzyme activity obtained was 879,47 UI/ml. This result will pave the way for further studies such as investigating the storage conditions of raw cellulase products obtained from this strain of bacteria, conducting fermentation studies on pilot and industrial scale to obtain cellulase in large quantities and quality, production of commercial enzyme products or applications of cellulase for a variety of practical purposes.