A STUDY OF FORMULATION AND CHARACTERIZATION OF NANO LIPOSOMES FROM Cordyceps militaris EXTRACTS USING MEMBRANE HYDRATION

Abstract

In this study, a simple procedure for the preparation of nano liposomes encapsulating extracts from Cordyceps militaris using the membrane hydration method was performed. The extraction of the mushroom extract was carried out using an ultrasound-assisted method with water as the solvent, and the extract was encapsulated using nano liposomes made from lecithin. Several factors influencing encapsulation efficiency and particle size of the nano system were investigated. The characteristics of the nano system were determined using techniques such as Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM), and Zeta potential analysis. Results indicated that the optimal lecithin concentration was 1:40 (w/v), with encapsulation efficiencies for the active compounds ADE reaching 73.3 ± 1.5% and COR reaching 76.0 ± 5.9%. The optimal homogenization power was found to be 12,000 rpm, with a homogenization time of 10 minutes. The resulting nano liposome system achieved an average particle size of 128.3 ± 4.2 nm, with a Zeta potential of -24.5 mV. The findings of this study highlight the potential for diversifying products derived from Cordyceps militaris to enhance the value of this precious medicinal mushroom.